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KMID : 0614620040430050291
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Korean Journal of Gastroenterology
2004 Volume.43 No. 5 p.291 ~ p.298
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Role of Cyclooxygenase-2 (COX-2) and Peroxisome Proliferator-Activated Receptor (PPAR) in Gastric Cancer
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Chang Young-Woon
Cho Hwoang-Lae
Jang Jae-Young
Dong Seok-Ho
Kim Hyo-Jong
Kim Byung-Ho
Lee Joung-Il
Chang Rin
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Abstract
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Background/Aims: Gastric cancer is still the most frequently diagnosed malignancy in Korea. It has been reported that COX-2 and PPAR are involved in multi-step gastric carcinogenesis. The aim of the present study was to examine the expression of COX-2 and PPAR in gastric cancer.
Methods: A total of 75 subjects including 45 patients with gastric cancer and 30 controls were enrolled. All subjects underwent upper gastrointestinal endoscopic examination with tissue collection. mRNA extraction from the tissues and real-time PCR for COX-2, PPAR-δ, and PPAR-?were performed. Gastric mucosal concentration of PGE2, which is a final product of COX-2, and 15d-PGJ2, which is a ligand of PPAR-γ were measured by the enzyme immunoassay method.
Results: COX-2 mRNA expression was significantly higher in both early gastric cancer tissues (EGC, 8.32±4.84㎍/㎕, p＜0.005) and advanced gastric cancer tissues (AGC, 8.16±2.67㎍/㎕, p＜0.001) than in non-cancerous tissues of controls (3.46±1.72㎍/㎕). There was no significant difference of PPAR-δ and PPAR-γ mRNA expression between gastric cancer tissues and controls. Mucosal PGE2 concentration was significantly higher in both EGC tissues (5.31±0.49㎍/㎎ protein, p＜0.001) and AGC tissues (5.46±0.54㎍/㎎ protein, p＜0.001) than in non-cancerous tissues of controls (4.22±8㎍/㎎ protein). There was no significant difference of 15d-PGJ2 concentration between gastric cancer tissues and controls.
Conclusions: COX-2 overexpression and increased PGE2 concentration in gastric tissues may play an important role in gastric carcinogenesis. However, the role of PPAR (δ and γ) and 15d-PGJ2 in gastric carcinogenesis is uncertain. Further studies are needed.
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KEYWORD
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Gastric cancer, COX-2, PPAR, PGE2, 15d-PGJ2, 위암
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